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AFMフォース測定

フォーススペクトロスコピー測定の一例である、フォースカーブ(力-距離の曲線)。 原子間力顕微鏡で力を印加して、タイチンとよばれるタンパク質を引伸ばしてアンフォールドさせた。図の各ピークは、タンパク質中のひとつのドメインのアンフォールディングに対応している。

原子間力顕微鏡 (AFM)は、柔らかい生体材料(分子、細胞、組織など)から、ポリマーや硬い無機物にいたるまで、材料のメカニクスを測定できる強力なツールです。 カンチレバーのバネ定数に応じて、単体の分子をほどいたり (分子内相互作用)、または2つの分子を引き離したり(分子間相互作用)するような力をpNスケールで測定できます。

AFMに関する技術的なお問い合わせ
  • 1分子フォーススペクトロスコピー
  • pNの力の分解能で分子を引っ張る
  • ヤング率を測定するために、トリガー制御(フォースまたはデフレクション)をかけて材料を押す
  • フォース・クランピング
  • フォース・ランピング
  • 適切な溶液、温度で操作
  • ケミカルフォース顕微鏡
  • コロイドプローブAFM
  • モジュラータンパク質のほどけ・アンフォールディング; エネルギーランドスケープの測定・観測
  • DNA分子の解離・融解(B-S遷移)
  • ポリマーの立体構造の変化
  • 分子間の力を測定:リガンド-レセプタ対、抗体-抗原の結合、タンパク質-ミネラル
  • 官能基と表面間の特異性を測定
  • 凝着力を測定
  • 細胞(固定したもの・生きたもの)、ポリマー、ゲルのメカニカル測定

下のリストより技術資料(英文)のダウンロードをご利用いただけます。
日本語版をご希望の場合にはこちらからご連絡ください。

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