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AFMフォース測定

フォーススペクトロスコピー測定の一例である、フォースカーブ(力-距離の曲線)。 原子間力顕微鏡で力を印加して、タイチンとよばれるタンパク質を引伸ばしてアンフォールドさせた。図の各ピークは、タンパク質中のひとつのドメインのアンフォールディングに対応している。

原子間力顕微鏡 (AFM)は、柔らかい生体材料(分子、細胞、組織など)から、ポリマーや硬い無機物にいたるまで、材料のメカニクスを測定できる強力なツールです。 カンチレバーのバネ定数に応じて、単体の分子をほどいたり (分子内相互作用)、または2つの分子を引き離したり(分子間相互作用)するような力をpNスケールで測定できます。

AFMに関する技術的なお問い合わせ
  • 1分子フォーススペクトロスコピー
  • pNの力の分解能で分子を引っ張る
  • ヤング率を測定するために、トリガー制御(フォースまたはデフレクション)をかけて材料を押す
  • フォース・クランピング
  • フォース・ランピング
  • 適切な溶液、温度で操作
  • ケミカルフォース顕微鏡
  • コロイドプローブAFM
  • モジュラータンパク質のほどけ・アンフォールディング; エネルギーランドスケープの測定・観測
  • DNA分子の解離・融解(B-S遷移)
  • ポリマーの立体構造の変化
  • 分子間の力を測定:リガンド-レセプタ対、抗体-抗原の結合、タンパク質-ミネラル
  • 官能基と表面間の特異性を測定
  • 凝着力を測定
  • 細胞(固定したもの・生きたもの)、ポリマー、ゲルのメカニカル測定

下のリストより技術資料(英文)のダウンロードをご利用いただけます。
日本語版をご希望の場合にはこちらからご連絡ください。

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"Poly(acrylamide) films at the solvent-induced glass transition: adhesion, tribology, and the influence of crosslinking," A. Li, S. N. Ramakrishna, E. S. Kooij, R. M. Espinosa-Marzal, and N. D. Spencer, Soft Matter 8, 9092 (2012). https://doi.org/10.1039/c2sm26222c

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"Molecular shape and binding force of Mycoplasma mobile's leg protein Gli349 revealed by an AFM study," C. Lesoil, T. Nonaka, H. Sekiguchi, T. Osada, M. Miyata, R. Afrin, and A. Ikai, Biochem. Biophys. Res. Commun. 391, 1312 (2010). https://doi.org/10.1016/j.bbrc.2009.12.023

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"Sacrificial bonds in polymer brushes from rat tail tendon functioning as nanoscale velcro," T. Gutsmann, T. Hassenkam, J. A. Cutroni, and P. K. Hansma, Biophys. J. 89, 536 (2005). https://doi.org/10.1529/biophysj.104.056747

"Surface probe measurements of the elasticity of sectioned tissue, thin gels and polyelectrolyte multilayer films: Correlations between substrate stiffness and cell adhesion," A. J. Engler, L. Richert, J. Y. Wong, C. Picart, and D .E. Discher, Surf. Sci. 570, 142 (2004). https://doi.org/10.1016/j.susc.2004.06.179

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